Noemie Guirandy

Human activities lead to the release of pollutants in the environment. Although Ecological Risk Assessment (ERA) aims at assessing their effects on ecosystem, there is a lack of representativeness, as the current methodology does not include long-term effects and ability of organisms to adapt. We propose to improve the methodology through technical innovations. We have chosen to study the effects of ethynilestradiol (EE2), an endocrine disruptor found in environment rich in biodiversity such as mangrove. Therefore, EE4 aims to study long-term effects of EE2 on the Kryptolebias marmoratus, an endemic fish of American mangroves, using multigenerational approach. Oxford Nanopore Technology will be used, to study genetics and DNA methylation on the same individual. The results will lead to highlight biomarkers of long-term effects, which will be assessed in the field. This approach (laboratory vs. field) is rarely conducted in ERA, although this represents an essential verification point.