SpoT regulation during infectiON
Dr. Régis Hallez
Bacteria use a variety of sensory systems to monitor and respond adequately to fluctuations in their environment. These signaling pathways allow bacteria to rapidly reshape their metabolism and physiology which optimizes their chances of survival. One of the most ubiquitous mechanisms used by virtually all bacteria relies on the sharp accumulation of a second-messenger, the guanosine tetra- or penta-phosphate (collectively referred to as (p)ppGpp). Despite its importance on bacterial physiology, the regulation of the enzymes that produce and degrade this second messenger in response to environmental cues is still poorly understood in pathogens. In this project, I propose to identify new regulations of enzymes synthesizing and hydrolysing (p)ppGpp in Salmonella during macrophage infection.
• 2007-2010: B.Sc. in Biology, University of Namur, Belgium
• 2010-2012: M.Sc. in Biochemstry, Molecular and Cellular Biology, University of Namur, Belgium
• 2012: Internship of 5 months at the Centre for Bacterial Cell Biology (CBCB), Newcastle Upon Tyne, UK
Supervisors: Dr. Henrik Strahl and Dr. Leendert Hamoen
• 2012-2016: PhD in Microbiology, Research Unit in Biology of Microorganisms, University of Namur, Belgium
Supervisor: Dr. Régis Hallez
• 2017 – 2022: Postdoctoral Fellow at Imperial College London, UK then Harvard Medical School (HMS), Boston, USA
Supervisor: Dr. Sophie Helaine
• 2021 – Ronneau S, Hill PW, Helaine S. Antibiotic persistence and tolerance: not just one and the same. Curr Opin Microbiol. Dec; 64:76-81.
• 2021 – Hill PWS*, Moldoveanu AL*, Sargen M, Ronneau S, Glegola-Madejska I, Beetham C, Fisher RA, Helaine S. The vulnerable versatility of Salmonella antibiotic persisters during infection. Cell Host Microbe 2021 Dec 8; 29(12):1757-1773.
• 2019 – Ronneau S, Hallez R. Make and break the alarmone: regulation of (p)ppGpp synthetase/hydrolase enzymes in bacteria. FEMS Microbiol Rev. Jul 1;43(4):389-400.
• 2019 – Ronneau S, Caballero-Montes J, Coppine J, Mayard A, Garcia-Pino A, Hallez R. Regulation of (p)ppGpp hydrolysis by a conserved archetypal regulatory domain. Nucleic Acids Res. Jan 25;47(2):843-854.
• 2016 – Ronneau S, Petit K, De Bolle X, Hallez R. Phosphotransferase-dependent accumulation of (p)ppGpp in response to glutamine deprivation in Caulobacter crescentus. Nat Commun. Apr 25;7:11423.
Complete list of publications available on Pubmed